Morphological characterization and molecular-genetic identification of natural isolates of biotechnologically valuable of yeast-like fungi
Abstract
New strains of yeast-like fungi were isolated from the surface of leaves, berries and flowers of decorative, medicinal and fruit cultivars. Following morphological examination of yeast colonies and cells, physiological-biochemical characterization and molecular-genetical identification the cultures were referred to the folowinggenera: Cystofilobasidium, Rhodotorula, Sporobolomyces, Rhodosporidiobolus — sources of carotenoid pigments, Cryptococcus, Metschnikowia, Bullera — potential producers of extracellular polysaccharides, Dothiora — melanin synthesizers. The isolated strains in short-term perspective may be used in biotechnological industry and manufacturing of bioactive additives.
About the Authors
A. V. Kanterova
The Institute of Microbiology of National Academy of Sciences of Belarus
Belarus
Belarus
Kanterova Anna V.
Kuprevich str. 2, 220141, Minsk, Republic of Belarus
S. I. Leanovich
The Institute of Microbiology of National Academy of Sciences of Belarus
Belarus
Belarus
Leanovich Svetlana I.
Kuprevich str. 2, 220141, Minsk, Republic of Belarus
A. V. Savchik
The Institute of Microbiology of National Academy of Sciences of Belarus
Belarus
Belarus
Savchik Anastasia V.
Kuprevich str. 2, 220141, Minsk, Republic of Belarus
A. I. Ladutska
The Institute of Microbiology of National Academy of Sciences of Belarus
Belarus
Belarus
Ladutska Alena I. — Ph.D. (Biological)
Kuprevich str. 2, 220141, Minsk, Republic of Belarus
G. I. Novik
The Institute of Microbiology of National Academy of Sciences of Belarus
Belarus
Belarus
Novik Galina I. — Ph.D. (Biological)
Kuprevich str. 2, 220141, Minsk, Republic of Belarus
References
1. Satyanarayana T, Kunze G. (eds). Yeast Biotechnology: Diversity and Applications. Springer Sci. + Business Media B.V. 2009, 744 p.
2. Hernandez-Almanza A., Montaneza J.C., Aguilar-Gonzalez M.A. et all. Rhodotorulaglutinis as source of pigments and metabolites for food industry.Food Biosci. 2014, no. 5, pp. 64–72.
3. Bab’yeva, I.P. Metody vydeleniya i indentifikatsii drozhzhey. Spravochnoye posobiye. [Methods of isolation and identification of yeast.Reference manual]. M.: «Pishchevaya promyshlennost’», 1979, 120 p. (in Russian).
4. Lхoke, M. Extraction of genomic DNA from yeasts for PCR — based applications. Biotechniques. 2011, 50, no. 5, pp. 325–328.
5. Barnett, J.A. A new key to the yeasts / J.A. Barnett, R.J. Pankhurst, Amsterdam: North — Holland Publishing Company, 1974, 273 p.
6. The yeasts. A taxonomic study.Second and enlarged edition. Ed.: J. Lodder., Amsterdam: North — Holland Publishing Company, 1970, 1385 p.
7. Lusta K.A., Fikhte B.A. Metody opredeleniya zhiznesposobnosti mikroorganizmov [Methods for determining the viability of microorganisms]. Nauchnyy tsentr biologicheskikh issledovaniy AN SSSR v Pushchine, 1990. 186 p.
Review
For citations:
Kanterova A.V., Leanovich S.I., Savchik A.V., Ladutska A.I., Novik G.I. Morphological characterization and molecular-genetic identification of natural isolates of biotechnologically valuable of yeast-like fungi. Food Industry: Science and Technology. 2020;13(1):46-53. (In Russ.)
Views: 207
Email this article 